GB/T 16551-2008 Diagnostic techniques for classical swine fever(hog cholera)

Scope

This standard specifies the technical requirements for the clinical diagnosis, pathological diagnosis, and laboratory diagnosis of swine fever (CSF). The laboratory diagnosis methods mainly include: rabbit passive immunization test, immunohistochemical staining test, virus isolation and identification test, direct immunofluorescence antibody test, fluorescent antibody virus neutralization test, swine fever monoclonal antibody enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) diagnostic techniques.
This standard is applicable to the diagnosis of swine fever. Five methods, including rabbit passive immunization test, immunohistochemical staining test, direct immunofluorescence antibody test, and RT-PCR, are mainly used for the diagnosis of swine fever virus and antigen to detect infected and naturally infected pigs; swine fever monoclonal antibody ELISA and fluorescent antibody virus neutralization test are mainly used for the monitoring of swine fever antibodies and the evaluation of immunity. Among them, the monoclonal antibody ELISA is mainly used for the differential diagnosis of swine fever antibodies, which can distinguish between naturally infected pigs, vaccinated pigs, pigs with positive antibodies to strong and weak viruses, and pigs with negative antibodies to swine fever.

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