T/YNBX 051-2022 Technical Specifications for Inducing Double Haploid Wheat from a Distant Hybrid of Wheat and Corn
T/YNBX 051-2022
Basic Information
Scope
Scope: This document specifies the methods for inducing wheat to produce diploid and monoploid materials through distant hybridization with maize, including wheat emasculation, wheat-maize hybridization, wheat cultivation in the laboratory, grain dehulling and disinfection, monoploid embryo culture, monoploid seedling doubling, seedling hardening, seedling transplantation, and bagging of doubled haploid plants, as well as double haploid seed harvesting. This document is applicable to wheat-maize distant hybridization for inducing wheat monoploid breeding or creating double haploid (DH) genetic populations. Main technical contents: Material cultivation, wheat emasculation, wheat-maize hybridization, wheat cultivation in the laboratory, grain dehulling and disinfection, monoploid embryo culture, monoploid seedling doubling, seedling hardening, seedling transplantation, bagging of doubled haploid plants, and double haploid seed harvesting.