T/NAASS 046-2022 Active Group standards

T/NAASS 046-2022 Determination of residual amounts of butyl acetate and n-butanol in tylosin tartrate by gas chromatography

Publish Date: 2022-12-28 Implement Date: 2023-01-31 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/NAASS 046-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Animal husbandry and breeding

Publish Date: 2022-12-28

Implement Date: 2023-01-31

Scope

Scope: This document specifies the gas chromatography detection method for residual butyl acetate and n-butanol solvents in tylosin tartrate. This document is applicable to the determination of residual butyl acetate and n-butanol solvents in tylosin tartrate. Main technical content: 3. Principle: The gas chromatograph equipped with a hydrogen flame ionization detector is used to separate, analyze, and determine the residual butyl acetate and n-butanol solvents in tylosin tartrate, with quantification performed using the external standard method. 4. Instruments and equipment 4.1 Gas chromatograph: Equipped with a hydrogen flame ionization detector and a headspace sampler. 4.2 Electronic balance: With a sensitivity of 0.0001 g. 4.3 Volumetric flasks: 10 mL, 100 mL. 4.4 Pipettes: 10 mL. 4.5 Headspace vials: 20 mL. 5. Reagents 5.1 Butyl acetate: Chromatographically pure. 5.2 n-Butanol: Chromatographically pure. 5.3 N,N-dimethylformamide: Chromatographically pure. 5.4 Standard solution: Accurately weigh 0.05 g each of butyl acetate and n-butanol (with a precision of 0.0001 g) and place them in a 100 mL volumetric flask. Dissolve and dilute them with N,N-dimethylformamide to the mark, shake well, and then quickly and accurately transfer 10.00 mL to a 20 mL headspace vial, seal it, and use it as the standard solution. 6. Determination 6.1 Chromatographic conditions a) Chromatographic column: A capillary column with a polyethylene glycol stationary phase (50 m*0.32 mm, 1.0 μm). Unless otherwise specified, polarity-similar chromatographic columns with equivalent separation effects can be interchangeably used. b) Carrier gas: Nitrogen (purity > 99.999%). c) Carrier gas flow rate: 2.3 mL/min. d) Split ratio: 20:1. e) Additional flow control: 10.0 mL/min. f) Column temperature: Initial temperature 100 °C for 6 min, then increase at a rate of 30 °C/min to 160 °C and maintain for 2 min. g) Injection port temperature: 200 °C. h) Evapora