T/NAASS 059-2023 Active Group standards

T/NAASS 059-2023 The Competitive Enzyme-Linked Immunosorbent Assay (ELISA) Antibody Detection Technique Specification for Bovine Respiratory Syncytial Virus Disease

Publish Date: 2023-03-22 Implement Date: 2023-04-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/NAASS 059-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Animal husbandry and breeding

Publish Date: 2023-03-22

Implement Date: 2023-04-25

Scope

Scope: This document specifies the terms and definitions of antibody detection by competitive enzyme-linked immunosorbent assay (cELISA) for bovine respiratory syncytial virus (BRSV) disease, and applies to the detection of antibodies against BRSV using cELISA. Key technical content: 3. Terms and definitions: The following terms and definitions apply to this document. - Bovine respiratory syncytial virus (BRSV): A single-stranded negative-sense RNA virus belonging to the Paramyxoviridae family and the Pneumovirus genus. It can cause acute respiratory disease syndrome in dairy and beef cattle. - 3.2 Competitive enzyme-linked immunosorbent assay (cELISA): A method in which a known specific antigen is coated on a solid support, and the sample and enzyme conjugate are added. The corresponding antibody can compete with the antigen and enzyme conjugate on the support. Unbound materials and the competed enzyme conjugate are washed away, and the enzyme substrate is added to measure the antibody by a colorimetric reaction. 4. Preparation for the cELISA assay: - Experimental environment, instruments and equipment: Follow the instructions in the kit. - Preparation of washing solution and dilution solution: Follow the kit instructions. - Preparation of tracer working solution: Follow the kit instructions. - Dilution of samples: Collect 2–3 mL of bovine venous blood, allow it to clot naturally for 1–2 hours, and centrifuge at 1,500–2,000 rpm/min for 5 minutes to collect serum in a 1.5 ml centrifuge tube. - Operating procedures: 4.3.1 Plate preparation: Remove the microplate and mark the sample positions on the operation log. If only a portion of the microplate is used, remove only the number of strips sufficient for testing the samples. Unused microplate strips should be wrapped in aluminum foil and stored at 2–8 °C. 4.3.2 Sample addition: Dilute the serum samples to the concentration specified in the kit and add the samples to each well, following the w