GB/T 15773-1995
Replaced
GB/T 41689-2022
Active
National standards
GB/T 41689-2022 Soil quality—Direct extraction of soil DNA
GB/T 41689-2022 Soil quality—Direct extraction of soil DNA
Basic Information
Standard Code:
GB/T 41689-2022
Standard Type:
National standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
\nSoil and fertilizer
ICS Name:
Comprehensive Study of Soil Quality and Soil Science
Publish Date:
2022-10-12
Implement Date:
2023-05-01
Pages:
11 pages
Scope
This document describes a method for directly extracting DNA from soil samples, which is used to analyze the abundance and composition of soil bacterial communities based on various molecular biology techniques, including real-time quantitative PCR (qPCR). This method is mainly suitable for agricultural and forest soils. However, it may not be suitable for soils rich in organic matter (such as peat soils) or soils heavily contaminated by organic pollutants or heavy metals.
Directly extracting DNA from soil samples provides a unique perspective for studying the alpha and beta diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR amplification of soil DNA will help develop routine tools for monitoring microbial communities in soil environments.
Development Information
Referenced Standards
ISO 18400-206
Adopt standards
ISO 11063:2020
Related Standards
GB/T 18834-2002
Active
GB/T 18834-2002 Soil quality—Vocabulary
TD/T 1043.1-2013
Active
TD/T 1043.1-2013 Technical regulation on subsurface drainage and desalination soil—Part 1:Soil survey
TD/T 1043.2-2013
Active
TD/T 1043.2-2013 Technical regulation on subsurface drainage and desalination soil—Part 2:Planning design and construction
GB/T 32720-2016
Active
GB/T 32720-2016 Laboratory methods for determination of microbial soil respiration
GB/T 32721-2016
Active