T/YNRZ 019-2024 Technical Specifications for the Production of Tissue Culture Seedlings of Yellow Yam from Bulblets

Scope

Scope: This document specifies the preparation of culture medium mother solution, the preparation of culture medium growth regulators, the preparation of culture medium, inoculation, cultivation, transplantation of seedlings, and the output of seedlings in the production of tissue culture seedlings of bulbous yellow yam. This document is applicable to the production of tissue culture seedlings of bulbous yellow yam. Main technical content: 1. Terms and definitions: There are no terms or definitions that need to be defined in this document. 2. Preparation of culture medium mother solution: 2.1 Preparation method and storage of mother solution: The mother solution is prepared in strict accordance with the methods specified in GB/T 603, and the water used meets the specifications and test methods for analytical laboratory water in GB/T 6682. The commonly used reagents are prepared into a mother solution with a concentration 10-100 times higher than that required by the culture medium, and the mother solution is stored in a refrigerator at 4°C. 2.2 Preparation of macronutrient mother solution: Weigh 33 g of ammonium nitrate, 38 g of potassium nitrate, 3.4 g of potassium dihydrogen phosphate, 7.4 g of magnesium sulfate heptahydrate, and 8.8 g of calcium chloride dihydrate, respectively. Add a small amount of distilled water to each container and dissolve them thoroughly with a glass rod. Then, adjust the volume to 1000 ml in a volumetric flask and store them in a brown wide-mouth bottle with a label. 2.3 Preparation of micronutrient mother solution: Weigh 0.166 g of potassium iodide, 1.24 g of boric acid, 3.38 g of manganese sulfate tetrahydrate, 1.72 g of zinc sulfate heptahydrate, 0.05 g of sodium molybdate, 0.005 g of copper sulfate pentahydrate, and 0.005 g of cobalt chloride hexahydrate, respectively. Mix them in a beaker with a small amount of distilled water and dissolve them thoroughly. Then, adjust the pH to 5.5 and add water to 1000 ml in a volumetric flask. St

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