T/SDAA 0054-2021 Active Group standards

T/SDAA 0054-2021 Animal-based food: Detection of 12 β-receptor agonists using enzyme-linked immunosorbent assay (ELISA) method

Publish Date: 2021-12-13 Implement Date: 2022-01-14 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/SDAA 0054-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Animal and poultry products

ICS Name: Animal husbandry and breeding

Publish Date: 2021-12-13

Implement Date: 2022-01-14

Scope

Main Technical Content: Technical Specifications for the Detection of 12 Beta-Agonist Drugs in Animal Food by Enzyme-Linked Immunosorbent Assay (ELISA) 1 Scope This document specifies the technical specifications for the detection of total residues of 12 beta-agonist drugs in animal food by enzyme-linked immunosorbent assay (ELISA). This document is applicable to the determination of total residues of 12 beta-agonist drugs (clenbuterol, salbutamol, clenbuterol hydrochloride, terbutaline, tert-butyl alcohol, brombuterol, marbofloxacin, sibutramine, mabutramine, bromopride, cimetidine, and fenoterol) in pork, beef, and lamb. The minimum detection limit for the 12 beta-agonist drugs in pork, beef, and lamb is 1μg/kg. 2 Normative Reference Documents The content of the following documents is an integral part of this document through normative reference. Among them, the referenced documents with a specified date are applicable to this document only for the version corresponding to that date; the unspecified date referenced documents are applicable to this document with their latest version (including all amendment sheets). GB/T 6682 Specifications and Test Methods for Water Used in Analytical Laboratories GB/T 33411 General Guidelines for Enzyme-Linked Immunoassay Kits 3 Principle The method uses an indirect competitive ELISA. The microplate is coated with clenbuterol antigen. The residual beta-agonist drugs in the sample compete with the clenbuterol antigen on the enzyme plate for clenbuterol antibody. After adding the enzyme-labeled antibody, the color reagent produces a color reaction, and the termination solution terminates the reaction. The absorbance is measured at 450 nm using an enzyme reader. The absorbance value is negatively correlated with the total residues of the 12 beta-agonist drugs, and the residue quantity can be determined by comparing with the standard curve. T/SDAA 0054-2021 4 Reagents or Materials The following reagents, u