T/NAASS 058-2023 Active Group standards

T/NAASS 058-2023 The technical specification for antigen detection of bovine coronavirus sandwich enzyme-linked immunosorbent assay

Publish Date: 2023-03-22 Implement Date: 2023-04-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/NAASS 058-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Animal husbandry and breeding

Publish Date: 2023-03-22

Implement Date: 2023-04-25

Scope

Scope: This document specifies the terms and definitions of antigen detection by sandwich enzyme-linked immunosorbent assay (sELISA) for bovine coronavirus (BCV) in cattle farms in Ningxia. This document is applicable to the antigen detection of BCV by sELISA in cattle farms in Ningxia. Main technical content: 3. Terms and definitions The following terms and definitions are applicable to this document. Bovine coronavirus (BCV) BCV belongs to the Coronaviridae family and is an important pathogen causing diarrhea in newborn calves and respiratory infections in adult cattle. Sandwich enzyme-linked immunosorbent assay (sELISA) The sELISA method involves coating a known specific antibody on a solid support, adding the sample to be tested and an enzyme-labeled antibody specific to the antigen. The corresponding antigen can then specifically bind to the antibody on the support and the enzyme-labeled antibody. Unbound materials and enzyme-labeled antibodies are washed away, and an enzyme substrate is added to determine the antigen by a color reaction. 4. Preparation for sELISA Experimental environment, instruments and equipment, preparation of washing solution, preparation of dilution solution, and preparation of tracer working solution shall be carried out in accordance with the instructions of the reagent kit. The biosafety conditions of the experimental environment shall comply with GB/T 19489, and the distilled water used for dilution shall comply with GB/T 6682. Sampling Collect bovine fecal samples and add an equal volume of sample dilution solution. Let the samples settle naturally for 10 minutes without centrifugation. Then, extract the supernatant of the fecal solution into a 1.5 ml centrifuge tube. Operating procedures Before use, all reagents shall be restored to a temperature of 18–24 °C and gently shaken or rotated to mix them evenly. 4.3.1 Remove the microplate and mark the position of the samples on the operation record sheet. If o