T/BJZMXH 508-2024 Protocol for tissue culture and underforest cultivation of garlic rootstocks
T/BJZMXH 508-2024 Protocol for tissue culture and underforest cultivation of garlic rootstocks
Basic Information
Scope
Scope: This document specifies the content of tissue culture, understory planting and management, harvesting, and technical file management for honeysuckle. This document is applicable to the tissue culture and understory cultivation of honeysuckle. Main technical contents: Preface II1 Scope 12 Normative referenced documents 13 Terms and definitions 14 Tissue culture 14.1 Preparation work 14.2 Culture medium preparation 24.3 Explant selection and disinfection 24.4 Primary culture 34.5 Subculture 34.6 Rooting culture 34.7 Seedling cultivation 35 Understory planting and management 45 1. Forest land environment 45 2. Land preparation and fertilization 45 3. Understory planting and maintenance 45 4. Weeding 45 5. Drainage 45 6. Pest and disease control 46 Harvesting 46.1 Harvesting period 46.2 Harvesting methods 57 Technical file management 5 Appendix A (informative) Formula table of culture medium and hormone dosage for each stage of honeysuckle tissue culture 6