T/GXAS 226-2021 Active Group standards

T/GXAS 226-2021 Rice seed carrying bacterial leaf blight and bacterial grain blight synchronous molecular detection protocol

Publish Date: 2021-09-13 Implement Date: 2021-09-20 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/GXAS 226-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: Plant cultivation

Publish Date: 2021-09-13

Implement Date: 2021-09-20

Scope

Scope: This document establishes the procedure for simultaneous detection of bacterial stripe disease bacteria (Xanthomonas oryzae pv. oryzicola (Xcola)) and bacterial grain dryness disease bacteria (Burkholderia glumae) in rice seeds by multiplex PCR technology, defines the related terms and definitions, provides information on the principles, instruments and reagents, culture media and solution preparation, and describes the operational instructions for sampling, sample processing and separation methods, multiplex PCR detection, result determination, and sample and utensil handling. This document is applicable to the simultaneous molecular detection of bacterial stripe disease bacteria and bacterial grain dryness disease bacteria in rice seeds within the administrative area of Guangxi. Main technical content: This document establishes the procedure for simultaneous detection of bacterial stripe disease bacteria (Xanthomonas oryzae pv. oryzicola (Xcola)) and bacterial grain dryness disease bacteria (Burkholderia glumae) in rice seeds by multiplex PCR technology, defines the related terms and definitions, provides information on the principles, instruments and reagents, culture media and solution preparation, and describes the operational instructions for sampling, sample processing and separation methods, multiplex PCR detection, result determination, and sample and utensil handling. This document is applicable to the simultaneous molecular detection of bacterial stripe disease bacteria and bacterial grain dryness disease bacteria in rice seeds within the administrative area of Guangxi.