T/GDP 021-2020 Antimicrobial Peptide Activity Detection Technical Specification
T/GDP 021-2020 Antimicrobial Peptide Activity Detection Technical Specification
Basic Information
Scope
Main technical content: ① Raising insects ② Inducing antibacterial activity in haemolymph using Escherichia coli, deltamethrin, insect physiological saline, ultraviolet rays, and ultrasound as induction sources ③ Analyzing the active components and purifying them using conventional gel chromatography and ion exchange chromatography based on their molecular weight and isoelectric point ④ Determining the antibacterial activity of the antibacterial peptides (agar well method) ⑤ Measuring the minimum inhibitory concentration (MIC) of the antibacterial peptides ⑥ Determining the antibacterial kinetics of the antibacterial peptides ⑦ Studying the effects of the antibacterial peptides on cell walls ⑧ Conducting a leakage test for intracellular ultraviolet-absorbing substances