GB/T 14551-1993
Replaced
T/GZTPA 0002-2019
Active
Group standards
T/GZTPA 0002-2019 Announcement on the Release of Determination of Chlorpyrifos Residue in Vegetables and Fruits Using Enzyme-Linked Immunosorbent Assay (ELISA) Method
T/GZTPA 0002-2019 Announcement on the Release of Determination of Chlorpyrifos Residue in Vegetables and Fruits Using Enzyme-Linked Immunosorbent Assay (ELISA) Method
Price:
$ 30
Basic Information
Standard Code:
T/GZTPA 0002-2019
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Integrated agriculture and forestry
ICS Name:
Integrated agriculture and forestry
Publish Date:
2019-12-12
Implement Date:
2020-01-01
Scope
Scope: This standard specifies the enzyme-linked immunosorbent assay (ELISA) method for determining the residual amount of chlorpyrifos in vegetables and fruits. This standard is applicable to the determination of the residual amount of chlorpyrifos in vegetables and fruits; Main technical content: The indirect competitive ELISA method is used. The conjugated antigen is coated on the microwell strips. The residual chlorpyrifos in the sample competes with the conjugated antigen on the enzyme plate for the chlorpyrifos antibody. After adding the enzyme-labeled antibody, the color reagent turns color, and the termination solution terminates the reaction. The absorbance is measured at 450 nm using an enzyme reader. The absorbance value is negatively correlated with the residual amount of chlorpyrifos. By comparing it with the standard curve and multiplying it by the corresponding dilution factor, the residual content of chlorpyrifos can be obtained.
Development Information
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