T/LYCY 1053-2023 Lupine detoxification and seedling technology regulations
T/LYCY 1053-2023 Lupine detoxification and seedling technology regulations
Basic Information
Scope
Scope: This document specifies the selection and pretreatment of mother plants for the cultivation of detoxified seedlings of Arundo donax, as well as the techniques for inoculation and cultivation, seedling hardening, and transplantation. This document is applicable to the cultivation of detoxified seedlings of Arundo donax in adapted regions. Main technical content: 4. Selection and pretreatment of mother plants 4.1 Selection of mother plants 4.2 Pretreatment 5. Inoculation and cultivation 5.1 Stripping of microshoot tips 5.2 Culture medium 5.3 Inoculation 5.4 Primary induction 5.5 Subculture and proliferation 5.6 Virus detection 5.7 Rooting cultivation 6. Seedling hardening and transplantation 6.1 Seedling hardening 6.2 Transplantation 6.3 Management