T/CI 019-2022 Cat rapid point-of-care testing guideline (fluorescent immunochromatographic assay)
T/CI 019-2022 Cat rapid point-of-care testing guideline (fluorescent immunochromatographic assay)
Basic Information
Scope
Scope: This guideline specifies the experimental condition requirements, experimental design, implementation and verification of rapid in vitro diagnostic techniques for cats, as well as related technical requirements such as instruments and detection principles. This guideline can serve as a reference document for detecting corresponding physiological or pathological indicators in cats, and is applicable to relevant personnel in research and development units of rapid in vitro diagnostic techniques for cats, pet hospitals, third-party testing centers, and pet grooming salons. Main technical content: 4.1 Samples and sample processing methods The samples used in the fluorescence immunoassay for cats mainly include: blood (whole blood, serum, plasma), secretions, and feces. 4.1.1 Blood Common blood collection sites for cats include the lateral small saphenous vein of the hind limb. When collecting blood, the cat is placed in a side-lying position or fixed on a cat stand, and the fur at the blood collection site is clipped. A latex tube is tied to the cat's thigh to expose the lower limb vein, and the assistant fixes the dog. Then, the blood collection site is disinfected with iodine solution, and the alcohol is used to decontaminate it to make the lower limb vein fill up. The left hand holds the blood collection hind limb, and the right hand holds the syringe with a needle attached to quickly puncture the vein. The needle is fixed with the left hand finger at an appropriate speed. 4.1.1.1 Whole blood without any additives: Use a pipette to measure an appropriate amount of freshly collected whole blood and add it to the designated diluent. After shaking it up and down, it can be used. For some items that do not require dilution, it can be used directly. Whole blood collected at room temperature should be tested as soon as possible and should not be stored for later use. Anticoagulated whole blood: Anticoagulated whole blood refers to whole blood sample