T/HPCIA 002-2023 Active

Scope: This document specifies the terms and definitions, raw materials, instruments and reagents, reagent preparation and preparation of test organisms, test methods, verification of test validity, result reporting, and interpretation of result relevance for the zebrafish stinging test method. This document provides a zebrafish biological evaluation method for the soothing efficacy of cosmetics, suitable for evaluating the efficacy of cosmetics that achieve soothing effects by alleviating stinging sensations. Note: The efficacy testing of cosmetic raw materials and compound raw materials can refer to this document. Main technical content: Foreword 1  Scope 2  Normative reference documents 3  Terms and definitions 4  Principle 5  Instruments and reagents 6  Reagent preparation and preparation of test organisms 7  Test methods 8  Verification of test validity 9  Result reporting 10  Interpretation of result relevance