T/HBAS 015-2023 Active

Scope: This document describes the method of determining capsaicin in edible oil by magnetic microparticle chemiluminescence. This document is applicable to the determination of capsaicin in edible oil; Main technical content: This method uses the principle of chemiluminescence competition. After the capsaicin in the sample is hydrolyzed, acidified, extracted, and redissolved, it competes with the antigen marker in the reagent kit for binding to the capsaicin-specific antibody conjugated to the magnetic microparticle carrier, or with the antigen conjugated to the magnetic microparticle carrier for binding to the capsaicin-specific antibody marker, forming an antigen-antibody complex. After magnetic separation and washing, the corresponding relative light units (RLU) are measured by an automatic chemiluminescence analyzer, and the amount of capsaicin in the sample is inversely proportional to the RLU value.