GB 7916-1987
Active
T/HPCIA 004-2021
Active
Group standards
T/HPCIA 004-2021 Acute toxicity of cosmetics determination using zebrafish embryos method
T/HPCIA 004-2021 Acute toxicity of cosmetics determination using zebrafish embryos method
Price:
$ 20.00
Basic Information
Standard Code:
T/HPCIA 004-2021
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Daily chemical products
ICS Name:
Cosmetics and beauty products
Publish Date:
2021-05-07
Implement Date:
2021-05-07
Scope
Scope: This standard specifies the methodological principles and operating procedures for evaluating the acute toxicity of cosmetics in zebrafish embryo experiments. This standard is applicable to the evaluation of the acute toxicity of cosmetics (creams, lotions, gels, serums, etc.) and cosmetic raw materials; Main technical content: Using a 6-well cell culture plate, under the control of a blank control or solvent control, zebrafish embryos aged 6–8 hours post-fertilization (hpf) are placed in samples of different concentrations and cultured at 28±0.5°C for 72 hours. Based on the toxicity response of the embryos to the test samples (embryo survival, development, and malformation), the cumulative survival rate, cumulative hatching rate, and cumulative malformation rate are calculated to characterize the acute toxicity of the samples.
Development Information
Related Standards
GB 7919-1987
Abolished
GB 7919-1987 Procedures and methods of safety evaluation for cosmetics
GB/T 7917.1-1987
Active
GB/T 7917.1-1987 Standard methods of hygienic test for cosmetics Mercury
GB/T 7917.2-1987
Active
GB/T 7917.2-1987 Standard methods of hygienic test for cosmetics Arsenic
GB/T 7917.3-1987
Active
GB/T 7917.3-1987 Standard methods of hygienic test for cosmetics Lead
GB/T 7917.4-1987
Active