T/NXSLX 0905-2024 The content of chlorogenic acid in the stems and leaves of Honeysuckle is measured as a standard

Scope

Scope: This document specifies the terminology, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of chlorogenic acid in honeysuckle stems and leaves in Ningxia region. This document is applicable to the determination of chlorogenic acid content in honeysuckle stems and leaves in Ningxia region. The detection limit is 57340 μg/mL. Main technical content: High-performance liquid chromatography is based on classical liquid chromatography, with liquid as the mobile phase. Different polar solvents are pumped into a column filled with highly fine particles of the fixed phase, where they are separated and detected by the detector, thereby achieving rapid and highly sensitive detection of samples. This method is used to detect and confirm the chlorogenic acid in honeysuckle stems and leaves. 7.1 Sample preparation: Take honeysuckle stem and leaf samples and crush them in a multifunctional crusher. Pass them through a 20-mesh sieve for later use. 7.2 Preparation of liquid solutions: 7.2.1 Preparation of standard solution: Accurately weigh 3.4 mg of chlorogenic acid standard and dissolve it in 50% methanol to a 10 mL volumetric flask, obtaining a 0.34 mg/mL chlorogenic acid standard control solution. 7.2.2 Preparation of sample solution: Precisely weigh 10 g of sieved honeysuckle powder, add 8 times the amount of water, soak for 30 minutes, boil for 30 minutes, filter, add 7 times the amount of water to the residue, boil for another 30 minutes, combine the filtrates, and concentrate them with a rotary evaporator. Dry them in a vacuum freeze-dryer to obtain honeysuckle extract. Precisely weigh 50 mg of honeysuckle extract, dissolve it in 50% methanol to a 10 mL volumetric flask, and filter it through a 0.22 um filter membrane to obtain the sample solution. 7.3 Sample determination: Inject 20μL of the sample solution under the following conditions: mob

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