T/XZYC 0009-2021 Technical Specifications for the Rapid Propagation of Polygonatum Odoratum by Tissue Culture

Scope

Main technical content: Preface 1 Scope 2 Normative reference documents 3 Terms and definitions 4 Main instruments and equipment 4.1 Sterilization equipment 4.2 Inoculation equipment 4.3 Cultivation equipment 4.4 Laboratory equipment 5 Preparation of culture media 5.1 Preparation of MS culture media 6 Inoculation and cultivation of explants 6.1 Selection of explants 6.2 Preparation before inoculation 6.3 Disinfection and sterilization of explants 7 Induction of differentiation, proliferation, and rooting cultivation 7.1 Induction of differentiation cultivation 7.2 Induction of proliferation cultivation 7.3 Induction of rooting cultivation 8 Environmental control of the cultivation room 9 Seedling acclimatization 9.1 Seedling acclimatization standards 9.2 Acclimatization of tissue-cultured seedlings 10 Transplantation and seedling cultivation 10.1 Preparation of substrate 10.2 Transplantation of seedlings 10.3 Management during the seedling stage 10.4 Criteria for seedling emergence 11 Packaging, labeling, and transportation 11.1 Packaging 11.2 Labeling 11.3 Transportation 12 File management Appendix A (Normative) MS culture medium stock solution component table Appendix B (Normative) 1/2 MS culture medium stock solution component table

Development Information

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