T/CAB CASA0002-2018 Determination of selenocysteine in selenium-rich agricultural products using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method
T/CAB CASA0002-2018 Determination of selenocysteine in selenium-rich agricultural products using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method
Basic Information
Scope
Scope: This standard specifies a method for determining the content of selenocysteine in agricultural products by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), which is suitable for the determination of selenocysteine content in selenium-rich agricultural products, such as grains, vegetables, fruits, etc. Main technical content: After sample processing, the test solution is separated into three forms of selenocysteine by high-performance liquid chromatography, and then each of them is introduced into the inductively coupled plasma mass spectrometer. Under the high-temperature action of the plasma, the sample is subjected to solvent removal, atomization, and ionization before entering the detector. Within a certain concentration range, the elemental signal intensity (CPS) value is proportional to the concentration of the analyte in the sample. By measuring the CPS value of the three forms of selenocysteine in the sample, its content can be calculated.