T/SQIA 009-2022 Rapid detection of imidacloprid in fruits and vegetables using colloidal gold immunochromatography method

Scope

Main technical content: This document specifies a rapid detection method for indoxacarb residues in vegetables and fruits using colloidal gold immunoassay. This document is applicable to the rapid detection of indoxacarb residues in vegetables and fruits. Note 1: Vegetables include head cabbage, cauliflower, broccoli, arugula, Chinese cabbage, spinach, common cabbage, leaf lettuce, head lettuce, Chinese white cabbage, solanaceous vegetables, and mung beans. Note 2: Fruits include pears, stone fruits, blueberries, grapes, and kiwis. This method adopts the principle of competitive inhibition immunoassay. After extraction, indoxacarb in the sample combines with the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color intensity of the detection line (T line). By comparing the color intensity of the detection line (T line) with that of the control line (C line), the indoxacarb residues in the sample can be qualitatively analyzed.

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