T/SQIA 008-2022 Rapid detection of hymexazol in fruits and vegetables using colloidal gold immunochromatography method

Scope

Main technical content: This document specifies a rapid detection method for the residues of chlorpyrifos in vegetables and fruits using colloidal gold immunoassay. This document is applicable to the rapid detection of chlorpyrifos residues in vegetables and fruits. Note 1: Vegetables include scallions, cabbage, cauliflower, broccoli, kohlrabi, Chinese cabbage, leaf vegetables, stem lettuce, cabbage, tomatoes, peppers, bell peppers, okra, stem lettuce, radishes, carrots, and turnips. Note 2: Fruits include citrus fruits, pome fruits, apples, peaches, nectarines, blueberries, cranberries, gooseberries, grapes, kiwis, avocados, papayas, and melons. This method uses the principle of competitive inhibition immunoassay. After extraction, the chlorpyrifos in the sample binds to the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color of the detection line (T line). By comparing the color of the detection line (T line) with that of the control line (C line), the chlorpyrifos in the sample can be qualitatively analyzed.

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