T/CQEEMA 11-2025 Soil and sediment—Determination of 3 chloramphenicol antibiotics —Liquid chromatography-triple quadrupole mass spectrometry

Scope

The "Determination of Three Chloramphenicol Antibiotics in Soil and Sediment by Liquid Chromatography-Triple Quadrupole Mass Spectrometry" aims to fill the technical gap in the monitoring of new pollutants in soil and support the implementation of the "List of Key Controlled New Pollutants (2023 Edition)". The document targets three target compounds, chloramphenicol, amoxicillin, and florfenicol, and innovatively adopts pressurized fluid extraction technology. The three chloramphenicol antibiotics in soil and sediment are extracted with methanol-citrate buffer solution, purified and concentrated by HLB extraction columns, and finally determined by liquid chromatography-triple quadrupole mass spectrometry. They are identified based on retention time and characteristic ion abundance ratio, and quantified by internal standard method. When the sampling amount is 1.0 g, the detection limit is 0.4 μg/kg~0.6 μg/kg, and the determination limit is 1.6 μg/kg~2.4 μg/kg. The document fills the detection gap of three chloramphenicol antibiotics in soil and sediment media, enhances the universality and accuracy of new pollutant monitoring, and provides standard technical support for environmental risk assessment and pollution control.

Development Information

Related Standards