T/ZJATA 0029-2025 Determination of Vitamin C, Isovitamin C, and Vitamin C Magnesium Phosphate in Beverages by High-Performance Liquid Chromatography

Scope

Main technical content: This standard includes the principles of testing methods for vitamin C, modified vitamin C, and vitamin C phosphate magnesium in beverages, reagents or materials, instruments and equipment, analysis steps, quality control, data processing, and precision. It specifies the high-performance liquid chromatography method for determining vitamin C, modified vitamin C, and vitamin C phosphate magnesium in beverages. After the beverage samples are dissolved and extracted by ultrasound with an appropriate amount of mobile phase, the method uses 0.01 mol/L ammonium hydrogen phosphate (pH=2.6) as the mobile phase, separates them through a C18 column, and detects them with a diode array or ultraviolet detector. It uses high-performance liquid chromatography to separate them, qualitatively analyzes them based on the retention time of the chromatographic peaks, and quantitatively analyzes them based on the peak area using the external standard method. The standard is advanced and innovative, and it meets the key development directions of group standard construction.

Development Information

Related Standards