T/DALN 039-2025 Active Group standards

T/DALN 039-2025 Diagnostic Technical Guidelines for Bovine Viral Diarrhea and Mucosal Diseases

T/DALN 039-2025 Diagnostic Technical Guidelines for Bovine Viral Diarrhea and Mucosal Disease

Publish Date: 2025-05-16 Implement Date: 2025-05-31 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/DALN 039-2025

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: -

ICS Name: Terminology (principles and coordination and cooperation)

Publish Date: 2025-05-16

Implement Date: 2025-05-31

Scope

Main Technical Content: 3.1 Bovine Viral Diarrhea - Mucosal Disease Bovine Viral Diarrhea - Mucosal Disease (BVD-MD) has a high incidence rate. It is caused by the Bovine Viral Diarrhea Virus (BVDV) and is a major acute, febrile infectious disease in cattle, with a certain mortality rate and the presence of persistent infection and immune suppression, causing significant harm to the cattle industry. 3.2 Clinical Symptoms of BVD-MD The main clinical symptoms of BVD-MD include diarrhea, high fever, and mucosal ulcers. The digestive tract shows linear lesions, accompanied by lethargy, anorexia, respiratory system diseases, and digestive system diseases. Severe cases may develop bloody stools, and the incubation period is typically 5-7 days. It can be divided into three types: acute type, persistent infection type (PI), and mucosal disease (MD). 4. Principles 4.1 Competitive Fluorescent Antibody Detection This method uses fluorescein-labeled antibodies to specifically bind to the target antigen on smears or tissue sections. A high-efficiency point light source is used to emit light of a specific wavelength through a filter plate, which excites the fluorescein bound to the specimen and produces fluorescence. The fluorescence staining pattern on the substrate can be observed under a fluorescence microscope to determine the presence of the target antigen or antibody. 4.2 Immunoperoxidase Monolayer Cell Test (IPMA) The main principle of this test is that the specific color reaction produced by the interaction of antigen and antibody can be observed and judged by the naked eye through an optical microscope. 4.3 Micro-Serum Neutralization Test After an animal is infected with a virus, specific neutralizing antibodies are produced in its body and bind specifically to the corresponding virus particles, thereby preventing the virus from adhering to sensitive cells or invading them, rendering the virus incapable of infection. The neutralization test is based