T/CAICC 05-2021 Active Group standards

T/CAICC 05-2021 Rapid detection of phorate in Chinese medicinal materials using colloidal gold immunochromatography method

Publish Date: 2022-01-01 Implement Date: 2022-01-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CAICC 05-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Integrated agriculture and forestry

ICS Name: Chemical laboratories, laboratory equipment

Publish Date: 2022-01-01

Implement Date: 2022-01-01

Scope

Scope: This method specifies a rapid detection method for water amitraz in traditional Chinese medicines using colloidal gold immunoassay. This method is suitable for the rapid determination of water amitraz in traditional Chinese medicines such as Bupleurum, Angelica, Codonopsis, Radix isatidis, Licorice, and Astragalus. Main technical content: 3. Principle: This method adopts the competitive inhibition immunoassay principle. After extraction, water amitraz in the sample binds to the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color intensity of the detection line. By comparing the color intensity of the detection line with that of the control line (C line), the qualitative determination of water amitraz in the sample is carried out. 4. Reagents and materials 4.1 Reagents: Unless otherwise specified, all reagents are of analytical grade, and the laboratory water should meet the requirements of Grade II water in GB/T 6682. 4.1.1 Methanol (CH3OH). 4.1.2 Extraction solution: Provided by the colloidal gold immunoassay test strip or prepared according to its instructions. 4.1.3 Reference material: The Chinese and English names, CAS registry number, molecular formula, and relative molecular mass of the water amitraz reference material are shown in Table 1. The purity should be ≥98%. Table 1: Chinese and English names, CAS registry number, molecular formula, and relative molecular mass of the water amitraz reference material Chinese name English name CAS registry number Molecular formula Relative molecular mass Isocarbophos Isocarbophos 24353-61-5 C11H16NO4PS 289.3 Note: Equivalent traceable substances can also be used. 4.2 Solution preparation 4.2.1 Water amitraz standard stock solution (100 μg/mL): Accurately weigh 1 mg of the water amitraz reference material (4.1.3) and place it in a 10 mL volum