GB 7916-1987
Active
T/HPCIA 007-2021
Active
Group standards
T/HPCIA 007-2021 The determination of cosmetics with anti-allergic effect using zebrafish embryos
T/HPCIA 007-2021 The determination of cosmetics with anti-allergic effect using zebrafish embryos
Price:
$ 20.00
Basic Information
Standard Code:
T/HPCIA 007-2021
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Daily chemical products
ICS Name:
Cosmetics and beauty products
Publish Date:
2021-05-07
Implement Date:
2021-05-07
Scope
Scope: This standard specifies the methodological principles and operational procedures for evaluating the anti-allergic properties of cosmetics using zebrafish embryo experiments. This standard applies to the evaluation of the anti-allergic properties of cosmetics (creams, lotions, gels, essences, etc.) and cosmetic raw materials. Main technical content: Using a 96-well cell culture plate, under the control of a blank control or solvent control, zebrafish embryos at 72 hours post-fertilization (hpf) are placed in the test samples pretreated for 2 hours. Compoud 48/80 is added to induce an allergic reaction, followed by the addition of BAPNA solution to each well. After incubation in a 37℃ constant temperature incubator for 24 hours, the absorbance values are measured using an enzyme analyzer, and the tryptase expression levels and inhibition rates of the test samples are calculated.
Development Information
Related Standards
GB 7919-1987
Abolished
GB 7919-1987 Procedures and methods of safety evaluation for cosmetics
GB/T 7917.1-1987
Active
GB/T 7917.1-1987 Standard methods of hygienic test for cosmetics Mercury
GB/T 7917.2-1987
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GB/T 7917.2-1987 Standard methods of hygienic test for cosmetics Arsenic
GB/T 7917.3-1987
Active
GB/T 7917.3-1987 Standard methods of hygienic test for cosmetics Lead
GB/T 7917.4-1987
Active