T/CSES 81-2023 Active Group standards

T/CSES 81-2023 Environmental DNA (eDNA) macrobarcode method for freshwater biota monitoring

Publish Date: 2023-01-04 Implement Date: 2023-01-04 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CSES 81-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Technical management

ICS Name: Integrated Environment and Environmental Protection

Publish Date: 2023-01-04

Implement Date: 2023-01-04

Scope

Scope: This document is applicable to the monitoring of a wide range of freshwater organisms based on DNA sequence similarity, including phytoplankton, attached algae, aquatic vascular plants, zooplankton, large benthic invertebrates, and fish, etc. Main technical content: The specific technical content includes: (1) Scope; (2) Normative reference documents; (3) Terminology and definitions; (4) Reagents; (5) Equipment and materials; (6) Environmental DNA macro-barcode monitoring methods; (7) Quality control and quality assurance; (8) Waste disposal; (9) Appendix A (informative) Environmental DNA fixatives; (10) Appendix B (informative) Environmental DNA sampling record forms; (11) Appendix C (informative) Methods for constructing cumulative curves of molecular taxa; (12) Appendix D (informative) Information on commonly used DNA barcode amplification primers; (13) Appendix E (informative) Molecular experiment record forms; (14) Appendix F (informative) Bioinformatics analysis record forms; (15) Appendix G (informative) Biostatistical tables; (16) Appendix H (normative) Field quality assurance; (17) Appendix I (normative) Laboratory quality assurance