T/JLFX 002-2022 Active Group standards

T/JLFX 002-2022 Technical Regulations for Laboratory Detection and Identification of Pine Wood Nematodes

T/JLFX 002-2022

Publish Date: 2022-05-07 Implement Date: 2022-05-07 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/JLFX 002-2022

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Plant quarantine, pest and disease control

ICS Name: Terminology (principles and coordination and cooperation)

Publish Date: 2022-05-07

Implement Date: 2022-05-07

Scope

Scope: This standard specifies the technical procedures for the molecular detection and identification of pine wood nematodes. This standard is applicable to the molecular detection and identification of pine wood nematodes and their host plants and products in the plant quarantine for import and export, domestic plant quarantine, and forestry pest surveys. Main technical content: (1) Standards and requirements for the detailed operation of pine wood nematode isolation. Pine wood nematodes are very small, and individual nematodes are difficult to observe with the naked eye during sampling. This standard specifies the standards and requirements for the isolation of pine wood nematodes in samples by standardizing the equipment needed for nematode isolation and the operation steps during nematode isolation, providing a reference for the actual operation of detection personnel. (2) Standards and requirements for the morphological identification of pine wood nematodes. To enhance the accuracy of pine wood nematode detection, this standard adds the operation instructions for the morphological identification of pine wood nematodes to guide pine wood nematode disease detection personnel in the morphological identification of pine wood nematodes, enhancing the accuracy and scientificity of laboratory identification of pine wood nematodes. (3) Standards and requirements for the use of corresponding equipment and operation procedures for fluorescence quantitative detection of pine wood nematodes. This standard specifies the corresponding steps of fluorescence quantitative detection of pine wood nematodes and the equipment parameter requirements during these steps.