T/SDAQI 044-2021 Active Group standards

T/SDAQI 044-2021 Detection of aflatoxins producing fungi in feed using real-time fluorescence PCR method

Publish Date: 2021-09-25 Implement Date: 2021-10-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/SDAQI 044-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: \nFeeds

Publish Date: 2021-09-25

Implement Date: 2021-10-25

Scope

Scope: Applicable to the detection of Aspergillus flavus and Parasporidium in plant-based feed raw materials that produce aflatoxin; Main technical content: The method uses the presence or absence of three key genes (the regulatory gene aflR, the oleandomycin methyltransferase Omt-1 gene, and the verotoxin A dehydrogenase Ver-1 gene) in the biochemical synthesis process of aflatoxin to determine whether the tested strain can produce aflatoxin. According to the formation mechanism of aflatoxin, aflatoxin can only be produced when all three key genes are present. In addition, the ITS sequence of the 5.8S rDNA, which is common to all fungi, is used as a positive reference gene to determine whether the extracted DNA template and the reaction can be successful.