T/NXSLX 0903-2024 Standard for Determining the Total Flavonoid Content in the Stems and Leaves of Honeysuckle

Scope

Scope: This document specifies the terms, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of total flavonoids in the stems and leaves of Houttuynia cordata in Ningxia region. This document is applicable to the determination of total flavonoid content in the stems and leaves of Houttuynia cordata in Ningxia region. The detection limit is 550 μg. Main technical content: The NaNO2-Al(NO3)3-NaOH colorimetric method is used to determine the total flavonoid content in the stems and leaves of Houttuynia cordata. In an alkaline nitrite solution, flavonoid compounds form a red complex with aluminum ions at 510 nm, and the absorbance value of the reaction product at 510 nm can be used to calculate the total flavonoid content in the sample. This method can be used to detect and confirm the total flavonoid content in the stems and leaves of Houttuynia cordata. 7. Determination steps 7.1 Sample preparation: Take Houttuynia cordata stems and leaves samples and grind them in a multifunctional grinder. Pass them through a 20-mesh sieve for use. 7.2 Sample extraction: Weigh and sieve 5 g of Houttuynia cordata stems and leaves (accurate to 0.0001 g) into a 250 mL round-bottomed flask. Add 44% ethanol at a ratio of 1:23, and ultrasonicate at 56°C and 100 Hz (250 W) for 43 minutes. Extract twice, combine the filtrates, and filter through a 0.22 μm filter membrane. The filtrate is the extraction solution of Houttuynia cordata stems and leaves. 7.3 Sample determination: Take 20 μL of the Houttuynia cordata stems and leaves extraction solution into a capped test tube, add 300 μL of 10% sodium nitrite solution, mix well, and incubate for 6 minutes. Then add 300 μL of 10% aluminum nitrate solution, incubate for 6 minutes, and finally add 4 mL of 10% sodium hydroxide solution and mix well. Finally, make up to 10 mL with deionized water. Take 200 μL of the rea

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