T/AHFIA 043-2019 Active Group standards

T/AHFIA 043-2019 Determination of α-Linolenic Acid and L-Phenyl Lactic Acid in Baijiu by High Performance Liquid Chromatography (HPLC) method

Publish Date: 2019-12-30 Implement Date: 2020-01-30 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/AHFIA 043-2019

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Food hygiene

ICS Name: Alcoholic beverages

Publish Date: 2019-12-30

Implement Date: 2020-01-30

Scope

Main Technical Content: **Main Provisions**: The chapters of this standard include scope, reagents and materials, main instruments, determination steps, result calculation, precision and detection limit, and method uncertainty. The main technical parameters and experimental validation parameters in this standard are as follows: 1. **Confirmation of Main Technical Parameters**: - **Selection of Chromatographic Columns**: Two different types of chromatographic columns were selected for testing: HSS T3 and BEH C18. Through a single-factor variable method, it was determined that the BEH C18 column performed better in separating α-linolenic acid and L-phenyl lactic acid, achieving complete separation. The chromatogram is shown in Figure 1. - **Selection of Mobile Phase**: Water-methanol, acetic acid-methanol, and water-acetonitrile were initially tested, but all showed poor separation results. It was found that using low concentrations of phosphoric acid significantly improved separation. Three phosphoric acid solutions (1%, 0.5%, and 0.1%) were tested as mobile phases, and the results showed that 0.1% phosphoric acid provided the best separation with good peak shapes, meeting the requirements. Therefore, 0.1% phosphoric acid was selected as the mobile phase for separating α-linolenic acid and L-phenyl lactic acid. - **Selection of Wavelength**: After literature review and experiments, the optimal detection wavelength of 205nm was determined through 3D scanning of the wavelength range of 210-500nm, followed by chromatogram extraction and literature reports. The standard sample validation confirmed the accuracy of this wavelength. 2. **Experimental Validation**: After confirming all technical parameters, the standard-setting team conducted precision, reproducibility, stability, and recovery rate tests to verify the effectiveness of the method. The relative standard deviations (RSD%) of parallel measurements were all less than 5%, meeting methodologi