T/HPCIA 006-2021 Active Group standards

T/HPCIA 006-2021 Cosmetics irritation test using zebrafish embryos

Publish Date: 2021-05-07 Implement Date: 2021-05-07 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/HPCIA 006-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Daily chemical products

ICS Name: Cosmetics and beauty products

Publish Date: 2021-05-07

Implement Date: 2021-05-07

Scope

Scope: This standard specifies the methodological principles and operating procedures for evaluating the mild irritancy of cosmetics using zebrafish embryo experiments. This standard applies to the evaluation of the mild irritancy of cosmetics (creams, lotions, gels, essences, etc.) and cosmetic raw materials; Main technical content: Using a 24-well cell culture plate, under the conditions of a blank control or a positive control, the 24-hour post-fertilization (hpf) zebrafish embryos are placed in different samples and observed under a stereomicroscope (within 5 minutes). Based on the mild irritancy response of the embryos to the test samples (the irritation swing of the embryos and the coagulation (death) situation), the number of embryo irritation swings (the movement of the yolk sac position) and the number of embryo deaths (coagulation) are recorded, characterizing the mild irritancy of the samples.