T/CAIA SH016-2021 Industrial cannabis Content determination of cannabidiol (CBD) and cannabidiolic acid using high-performance liquid chromatography (HPLC) method
T/CAIA SH016-2021 Industrial cannabis Content determination of cannabidiol (CBD) and cannabidiolic acid using high-performance liquid chromatography (HPLC) method
Basic Information
Scope
Scope: This document specifies the principles, instruments, reagents, analytical steps, instrument measurement conditions, result calculation and presentation, and data accuracy for determining cannabidiol and cannabidiolic acid in industrial hemp using high-performance liquid chromatography. This document is applicable to the determination of cannabidiol and cannabidiolic acid content in industrial hemp, with a quantitative measurement range of 0.4 mg/g to 100 mg/g. Main technical content: After drying, crushing, grinding, and sieving industrial hemp samples, they are extracted with a 95% ethanol solution, filtered with a microporous membrane, separated by reverse-phase chromatography, and detected by an ultraviolet detector. Quantification is performed using an external standard method.