T/GXAS 753-2024 The determination of steroidal hormones in seawater using high performance liquid chromatography-tandem mass spectrometry
T/GXAS 753-2024 The determination of steroidal hormones in seawater using high performance liquid chromatography-tandem mass spectrometry
Basic Information
Scope
Scope: This document specifies a high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in seawater. This document is applicable to the determination of 15 steroid hormones in seawater, including androgens (nandrolone, testosterone, methyltestosterone, androstenedione), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be measured after validation for applicability. When the sample volume is 1,000 mL, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method has a detection limit of 1.6 ng/L to 3.9 ng/L and a quantification limit of 6.4 ng/L to 15.6 ng/L, in accordance with Appendix A. Main technical content: This document specifies a high-performance liquid chromatography-tandem mass spectrometry method for the determination of steroid hormones in seawater. This document is applicable to the determination of 15 steroid hormones in seawater, including androgens (nandrolone, testosterone, methyltestosterone, androstenedione), progestogens (progesterone, norgestrel, medroxyprogesterone, mifepristone), glucocorticoids (cortisone, hydrocortisone, dexamethasone), and estrogens (estrone, 17-α-estradiol, 17β-estradiol, estriol). Other steroid hormones can be measured after validation for applicability. When the sample volume is 1,000 mL, the concentrated and diluted volume is 1.0 mL, and the injection volume is 5.0 μL, the method has a detection limit of 1.6 ng/L to 3.9 ng/L and a quantification limit of 6.4 ng/L to 15.6 ng/L, in accordance with Appendix A.