T/BTFDIA T15/BTFDIA002-2019 Non-prepackaged ready-to-eat food rapid detection method for pathogenic bacteria
T/BTFDIA T15/BTFDIA002-2019 Non-prepackaged ready-to-eat food rapid detection method for pathogenic bacteria
Basic Information
Scope
The main technical content is as follows: After enriching and culturing the pathogenic microorganisms in the sample and extracting their nucleic acids, the DNA fragments of the pathogenic microorganisms are used as templates. After amplification with primers labeled with biotin (digoxin) at both ends, the amplified sample solution flows downward along a membrane fixed with streptavidin (digoxin) and colloidal gold. When it reaches the detection line area coated with the corresponding antibody, the biotin (digoxin)-labeled antigen in the amplified sample solution specifically binds with streptavidin (digoxin), colloidal gold, and the corresponding antibody, causing the detection line to display a certain color, thereby achieving specific immunodiagnosis.