GB/T 18415-2001
Active
T/CAIA SH007-2017
Active
Group standards
T/CAIA SH007-2017 Food Vibrio Paratyphi A Real-Time Fluorescent Polymerase Chain Reaction Amplification of DNA at Constant Temperature
T/CAIA SH007-2017 Food Vibrio Paratyphi A Real-Time Fluorescent Polymerase Chain Reaction Amplification of DNA at Constant Temperature
Price:
$ 20.00
Basic Information
Standard Code:
T/CAIA SH007-2017
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
Food Composite
ICS Name:
General methods for food testing and analysis
Publish Date:
2017-07-20
Implement Date:
2017-10-01
Scope
Scope: This standard specifies the SAT testing method for Vibrio parahaemolyticus in food. This standard is applicable to the detection of Vibrio parahaemolyticus in aquatic products and related food poisoning samples. Main technical content: At the same temperature, using bacterial RNA as the starting template, the reverse transcriptase M-MLV is used to generate a double-stranded DNA of the target RNA. Then, T7 RNA polymerase is used to generate multiple (100–1000) RNA copies from this DNA as the template. Each RNA copy then enters the next amplification cycle from the reverse transcription. At the same time, the optimized probes with fluorescent markers specifically bind to these RNA copies, opening the stem-loop structure and generating fluorescence. Based on the appearance time and intensity of the real-time fluorescence signal, combined with positive and negative controls, the detection results can be determined.
Development Information
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