T/SQIA 007-2022 The rapid detection of isocyanide in fruits and vegetables using colloidal gold immunochromatography method

Scope

Main technical content: This document specifies a rapid detection method for boscalid residues in vegetables and fruits using colloidal gold immunoassay. This document is applicable to the rapid detection of boscalid residues in vegetables and fruits. Note 1: Vegetables include onions, chives, scallions, cauliflowers, spinach, leaf lettuce, Chinese chives, stem lettuce, tomatoes, peppers, cucumbers, beans, edible soybeans, stem lettuce, carrots, potatoes, watercress. Note 2: Fruits include apples, pears, hawthorns, loquats, quinces, peaches, cherries, blackberries, gooseberries, grapes, kiwis, bananas, watermelons, and cantaloupes. This method uses the principle of competitive inhibition immunoassay. After extraction, the boscalid in the sample binds to the specific antibody labeled with colloidal gold, inhibiting the binding of the antibody and the antigen on the detection line (T line) of the test strip, resulting in a change in the color of the detection line (T line). By comparing the color of the detection line (T line) with that of the control line (C line), the boscalid in the sample can be qualitatively analyzed.

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