GB/T 18415-2001
Active
T/CAIA SHO19-2022
Active
Group standards
T/CAIA SHO19-2022 Acetylcholinesterase activity detection spectrophotometry
T/CAIA SHO19-2022 Acetylcholinesterase activity detection spectrophotometry
Price:
$ 20.00
Basic Information
Standard Code:
T/CAIA SHO19-2022
Standard Type:
Group standards
Standard Status:
Active
is_force_gb:
no
CCS Name:
-
ICS Name:
General methods for food testing and analysis
Publish Date:
2022-10-25
Implement Date:
2022-12-01
Scope
Main technical content: Acetylcholinesterase can hydrolyze iodinated thioacetylcholine to produce thiocoline and acetic acid. Thiocoline reacts with the sulfhydryl color reagent 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) to form a yellow compound, 5-mercapto-2-nitrobenzoic acid (TNB), which has a characteristic absorption at a wavelength of 412 nm. The absorbance value is measured; according to the reaction between the standard solution prepared from reduced glutathione and DTNB, a standard curve of the relationship between the amount of glutathione and the absorbance is drawn, and the enzymatic activity value of acetylcholinesterase is calculated.
Development Information
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