T/HPCIA 005-2022 Determination of whitening efficacy of cosmetics using zebrafish embryotoxicity test

Scope

Scope: This standard specifies the methodological principles and operating procedures for evaluating the whitening efficacy of cosmetics using zebrafish embryo experiments. This standard applies to the evaluation of the whitening efficacy of cosmetics (creams, lotions, gels, serums, etc.) and cosmetic raw materials; Main technical content: Compared with T/HPCIA 005-2021, in addition to editorial amendments, the main technical changes are as follows: - The standard adds T/SHRH 021-2019, "Test Method for Whitening Efficacy of Cosmetics using an In Vitro Reconstituted 3D Melanin Model"; - The standard adds T/ZHCA 001-2018, "Test Method for Whitening and Freckle Removing Efficacy of Cosmetics"; - Deletes the first qualitative method; - Modifies the observation requirements of the melanin production gray detection method; - Deletes the selection of cell culture plates for the melanin production gray detection method; - Modifies the materials and preparation method of embryo buffer water; - Modifies the solution concentration and preparation method of the positive control group; - Modifies the embryo density in embryo preparation; - Modifies the experimental method of the melanin production gray detection method; - Modifies the quality control; - Modifies Appendix C, "Pre-treatment Methods for Cosmetics and Raw Materials"; - Deletes Appendix D, "Data Recording Form"; - Adds Appendix E, "Schematic Diagram of Zebrafish Top-Down Photography".

Development Information

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