T/SDAQI 043-2021 Active Group standards

T/SDAQI 043-2021 The rapid detection method for Salmonella, Vibrio parahaemolyticus, and Shigella triple fluorescence quantitative PCR in feed

Publish Date: 2021-09-25 Implement Date: 2021-10-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/SDAQI 043-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Agriculture, forestry

ICS Name: \nFeeds

Publish Date: 2021-09-25

Implement Date: 2021-10-25

Scope

Scope: Applicable to pet feed products; Main technical content: The samples are cultured with bacteria, and the cultured bacterial broth is collected for DNA extraction. Using DNA as a template, the bacterial 16SrDNA gene sequence is used as an internal reference. Primers and probes are designed based on the relatively conserved and highly specific sequences of the invA gene in Salmonella, the tlh gene in Vibrio parahaemolyticus, and the ipaH gene in Shigella for the triplex real-time fluorescence PCR amplification of the target. According to the Ct value, it is determined whether the samples contain Salmonella, Vibrio parahaemolyticus, and Shigella. Among them, the detection of the internal reference reaction can monitor whether the reaction is normal and prevent false negative results.