T/NXSLX 0901-2024 The content of oxymatrine in the stem and leaves of Platycodon grandiflorus was determined using a standard method
T/NXSLX 0901-2024 The content of oxymatrine in the stem and leaves of Platycodon grandiflorus was determined using a standard method
Basic Information
Scope
Scope: This document specifies the terms, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of Platycodin D in the stem and leaves of Platycodon grandiflorum in Ningxia region. This document is applicable to the determination of Platycodin D content in the stem and leaves of Platycodon grandiflorum in Ningxia region. The detection limit is 30180μg. Main technical content: The colorimetric principle of the vanillin - sulfuric acid method is to dehydrate the carboxyl group, increase the double bond structure, and then shift the double bond and undergo dimeric condensation to form a double bond system. Vanillin reacts with Platycodon grandiflorum saponin D to form a colored compound, and under the action of acid, a positively charged carbonate salt is formed to give a color. Through this method, Platycodon grandiflorum saponin D in the stem and leaves can be detected and confirmed.