T/SMSWYY 001-2022 "Technical Regulations for Seedling and Bionomic Cultivation of "Minghu 1" Dendrobium Cheilosiphon"
T/SMSWYY 001-2022 "Technical Regulations for Seedling and Bionomic Cultivation of "Minghu 1" Dendrobium Cheilosiphon"
Basic Information
Scope
Main Technical Content: 4.3.1 Seedling Fast Propagation Utilizing plant tissue culture technology to cultivate Dendrobium officinale seedlings, original bulb-induced seedlings, and adventitious bud-induced seedlings, which can be produced on a large scale. The original bulb should be subcultured for 6–8 generations, and the adventitious bud should be subcultured for 8–12 generations. 4.4 Seedling Transplantation 4.4.1 Seedling Selection When the rooted seedlings reach 4–6 leaves, a plant height of 2.5 cm or more, and at least 2 root systems, they can be transplanted. The selected seedlings should have a green leaf color, be well-spread, free from yellow leaves, and have no contaminating bacteria in the bottle. 4.4.2 Seedling Transplantation Place the tissue-cultured seedlings in a greenhouse for 3–7 days, then carefully remove them. After rinsing thoroughly with water, let them dry until the roots turn white. 4.4.3 Seedling Grading Seedling quality is graded into Class I and Class II. Quality standards are specified in Appendix A. 4.4.4 Quarantine The seedlings must undergo quarantine before export. Quarantine methods are implemented in accordance with GB 15569. 4.4.5 Packaging Seedlings should be placed upright in ventilated plastic baskets or cardboard boxes. Information such as seedling variety or parent source, propagation method (seedling or stem-induced seedling), quantity, batch number, origin, production unit, transplantation date, and seedling inspection certificate number must be labeled. 4.4.6 Transportation Seedlings should be transported in a ventilated and humid environment. 5 Transplantation and Planting 5.1 Greenhouse Cultivation 5.1.1 Facility Selection Glass greenhouses, standard greenhouses, or ordinary greenhouses can be used. Facilities such as shading nets, insect-proof nets, spraying, and irrigation systems should be installed. Before planting, clean the greenhouse and surrounding weeds, and spray or fumi