T/FJBR 006-2023 Rapid detection of 6-benzylaminopurine, 6-furfurylaminopurine, and N6-isopentenyladenine in bean sprouts using Raman spectroscopy
T/FJBR 006-2023 Rapid detection of 6-benzylaminopurine, 6-furfurylaminopurine, and N6-isopentenyladenine in bean sprouts using Raman spectroscopy
Basic Information
Scope
Scope: This document is applicable to the screening and detection of 6-benzylaminopurine, 6-furfurylaminopurine, and N6-isopentenyladenine in various sprout products such as mung bean sprouts and soybean sprouts. Main technical content: This document specifies the method of using Raman spectroscopy to detect 6-benzylaminopurine, 6-furfurylaminopurine, and N6-isopentenyladenine in sprout products. Different substances have Raman spectra corresponding to their molecular structures. When a nano-enhanced reagent with surface-enhanced Raman scattering (SERS) effect is introduced, the Raman signal of the substance will be significantly enhanced. Take an appropriate amount of crushed samples, extract, purify, and enrich them with solvents. Then, add a surface-enhanced Raman scattering (SERS) enhancing reagent to effectively enhance the Raman signal of the target substances, and conduct Raman spectroscopy scanning. According to the Raman characteristic spectral peaks of 6-benzylaminopurine, 6-furfurylaminopurine, and N6-isopentenyladenine (see Appendix A), the results are determined as follows: the presence of characteristic peaks indicates a positive result, and otherwise, it indicates a negative result.