T/CITS 0012-2023 Active Group standards

T/CITS 0012-2023 Determination of A1 and A2 β-casein in infant formula milk powder based on cow milk and cow milk by liquid chromatography-tandem mass spectrometry

Publish Date: 2023-07-06 Implement Date: 2023-08-01 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/CITS 0012-2023

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Milk and dairy products

ICS Name: Other dairy products

Publish Date: 2023-07-06

Implement Date: 2023-08-01

Scope

Scope: This regulation specifies the determination methods for A1β-casein and A2β-casein in milk-based infant and toddler formula milk powder and milk. This regulation is applicable to the determination of A1β-casein and A2β-casein in milk-based infant and toddler formula milk powder and liquid milk. It is not applicable to fermented dairy products and products containing hydrolyzed or enzymatically digested casein. Main technical content: Bovine β-casein is enzymatically digested into peptides by trypsin, separated by reversed phase chromatography, and detected by tandem quadrupole mass spectrometry. Through the screening of specific peptide segments, the content of bovine A1 and A2β-casein in the sample is calculated using stable isotope dilution internal standard method.