T/NXSLX 0906-2024 The standard for determining the content of trichotomolignane glucoside in the leaves of astragalus root

Scope

Scope: This document specifies the terms, definitions, principles, reagents, materials, instruments, equipment, sample collection and preparation, determination steps, and result calculation for the determination of isoflavone glucoside in Astragalus stem and leaf in Ningxia region. This document is applicable to the determination of isoflavone glucoside in Astragalus stem and leaf in Ningxia region. The detection limit is 7.03125112.5μg/mL. Main technical content: High-performance liquid chromatography is based on classical liquid chromatography. A liquid is used as the mobile phase, and different polar solvents are pumped into a high-efficiency stationary phase chromatographic column. The samples are separated in the chromatographic column and detected by the detector, thereby achieving rapid and highly sensitive detection of the samples. This method is used to detect and confirm isoflavone glucoside in Astragalus stem and leaf. 7.1 Sample preparation: Take Astragalus stem and leaf samples and grind them in a multifunctional crusher. Pass them through a 20-mesh sieve for storage. 7.2 Preparation of liquid solutions: 7.2.1 Preparation of standard solution: Precisely weigh 4.5 mg of isoflavone glucoside standard and place it in a 10 mL volumetric flask. Add methanol to the flask until it reaches the mark, thereby obtaining a 450 μg/mL isoflavone glucoside standard solution. 7.2.2 Preparation of sample solution: Precisely weigh 1 g of sieved Astragalus stem and leaf powder and place it in a round-bottomed flask. Add 50 mL of methanol, weigh the mixture, heat it for 4 hours, cool it, and then weigh it again. Add methanol to make up for the weight loss. Shake well and filter the solution through a 0.22μm filter membrane to obtain the sample solution. 7.3 Determination of samples: Draw 20 μL of the test solution and inject it under the following conditions: mobile phase of 0.2% formic acid (A) - acetonitrile (B) (0~8 min 80% A~60%), column temperature 30℃,

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