The qualitative detection method of rice-derived components in traditional Chinese medicines using PCR technology, as specified in T/FDSA 0080-2025
T/FDSA 0080-2025
Basic Information
Scope
Scope: This document describes the PCR qualitative detection method for detecting rice-derived components in traditional Chinese medicines. This document is applicable to the PCR qualitative detection of rice-derived components in traditional Chinese medicines such as Hejie Dingchuan Wan, Shenling Baizhu Wan, Ren Shen Jianpi Wan, Liwei Dihuang Wan, Gui Pi Wan, Niu Huang Jiedu Tablets, Bao He Wan, Huang Lian Shangqing Wan, Long Dan Xie Gan Wan, Feng Feng Tong Sheng Wan, Feng Han Ko Chou Wan, Tong Xuan Li Fei Wan, Ji Wei Qiang Huo Wan, etc. Main technical content: 5 Reagents and materials 6 Instruments and equipment 7 Sample selection and preparation 8 Detection method 8.1 DNA extraction from samples 8.2 Determination of DNA purity and concentration 8.3 PCR amplification 8.4 Electrophoretic detection of PCR amplification products 9 Result analysis, judgment, and expression 10 Measures to prevent cross-contamination during detection 11 Waste disposal