T/HPCIA 004-2022 Acute toxicity test of cosmetics using zebrafish embryos method

Scope

Scope: This standard specifies the methodological principles and operational procedures for evaluating the acute toxicity of cosmetics using the zebrafish embryo test. This standard applies to the acute toxicity evaluation of cosmetics (creams, lotions, gels, essences, etc.) and cosmetic raw materials; Main technical content: Compared with T/HPCIA 004-2021, in addition to editorial amendments, the main technical changes are as follows: - Deleting the definitions and explanations of cumulative hatching rate, cumulative malformation rate, cumulative mortality rate, and cumulative survival rate; - Adding the definition and explanation of mortality rate; - Modifying the methodological principles; - Modifying the requirements for materials on the perforated plate; - Modifying the composition and preparation method of embryo buffer water; - Deleting the positive control group; - Modifying the requirements for embryo density in embryo preparation; - Modifying the pre-experiment and exposure experiment methods in the experimental methods; - Deleting the quality control requirements for cumulative mortality rate; - Deleting the quality control requirements for cumulative hatching rate; - Adding the quality control requirements for mortality rate; - Modifying the determination indicators; - Modifying the result calculation; - Modifying Appendix D, Pre-treatment Methods for Cosmetics and Raw Materials; - Deleting Appendix E, Data Recording Form.

Development Information

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