T/SDAQI 005-2021 Active Group standards

T/SDAQI 005-2021 The rapid qualitative detection of Pseudomonas aeruginosa in cosmetics using real-time fluorescence PCR method

Publish Date: 2021-09-25 Implement Date: 2021-10-25 For services related to genuine standard inquiry, procurement, translation, and other related services in China, please Contact Us

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Basic Information

Standard Code: T/SDAQI 005-2021

Standard Type: Group standards

Standard Status: Active

is_force_gb: no

CCS Name: Light industry, culture, and daily necessities

ICS Name: Cosmetics and beauty products

Publish Date: 2021-09-25

Implement Date: 2021-10-25

Scope

Scope: This document is applicable to the rapid qualitative detection of Pseudomonas aeruginosa in cosmetics. Main technical content: After culturing the samples, the bacterial solution is collected for DNA extraction. Using DNA as a template, the bacterial 16S rDNA gene sequence is used as an internal reference. The highly conserved and highly specific primers and probes in the exotoxin A (eta) gene of Pseudomonas aeruginosa are used for real-time fluorescence PCR amplification of the target. Based on the Ct value, it is determined whether the sample contains Pseudomonas aeruginosa. Among them, the detection of the internal reference reaction can monitor whether the reaction is proceeding normally and prevent false negative results.