T/SSSC 016-2025 Soil environmental microplastic monitoring technical specification standard - Laser micro-Raman spectroscopy Fourier transform infrared spectroscopy-optical microscopy method

Scope

Main technical content: 1. After grinding the samples collected in the field, sieve them using a standard 5 mm pore-sized sieve, dry them at 60 °C for 24 hours, and take 30 g of the processed samples and place them in a beaker. 2. Prepare 200 mL of saturated sodium chloride solution as the flotation reagent and pour it into the beaker. 3. Stir the mixture with a magnetic stirrer for 30 minutes, ultrasonically treat it for 10 minutes, and let it stand for 24 hours. 4. Use a glass rod to drain the flotation reagent slowly along the inner wall of the large beaker to avoid splashing and disturbing the original solution. 5. During the process of pouring in the flotation reagent, the lower-density substances will flow out with the upper layer of the flotation reagent and be transferred to a nylon filter membrane with a pore size of 0.45 μm in a funnel. Extend the settling time to 12 hours and repeat steps 3, 4, and 5 four times. 6. Turn on the vacuum filtration pump to filter the upper layer solution and wash the funnel wall with ultrapure water. Continue to filter to remove the salt base. 7. Rinse the solid materials flotated on the filter membrane and digest them with 100 mL of 30% hydrogen peroxide at 50 °C and a shaking frequency of 150 rpm/min for 2 days. 8. After the digestion is complete, repeat step 6 to separate the microplastic particles.

Development Information

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